Group Publications
Dorrestein, P.C.; Van Lanen, S.G.; Li, W.; Zhao, C.; Deng, Z.; Shen, B.; Kelleher, N.L. “The Bifunctional Glyceryl Transferase/Phosphatase OzmB Belonging to the HAD Superfamily That Diverts 1,3-Bisphosphoglycerate into Polyketide Biosynthesis.” J. Am. Chem. Soc., 2006, submitted.
Chan, Y.A.; Boyne, M.; Podevels, A.; Klimowicz, A.K.; Handelsman, J.; Kelleher, N.L.; Thomas, M.T. “Formation of Two Unusual Polyketide Synthase Extender Units: Hydroxymalonyl-Acyl Carrier Protein and Aminomalonyl-Acyl Carrier Protein”, Proc. Natl. Acad. Sci. U.S.A., 2006, submitted.
Pesavento, J.P.; Kelleher, N.L.; Mizzen C.A. “Histone H4 from Normal
and Cancer Cells.” Nature Genetics, under review.
Garcia, B.A.; Siuti, N.; Thomas, C.E.; Mizzen, C.A.; Kelleher,
N.L. “Characterization of Neurohistone Variants and Post-translational
Modifications by Electron Capture Dissociation Mass Spectrometry,”
Int. J. Mass Spectrom., 2006, accepted.
(Special issue honoring Donald F. Hunt on his 65th Birthday.)
Garcia, B.A.; Joshi, S.; Thomas, C.E.; Chitta, R.K.; Diaz, R.L.;
Busby, S.A.; Andrews, P.C.; Ogorzalek Loo, R.R.; Shabanowitz,
J.; Kelleher, N.L.; Mizzen, C.A.; Allis, C.D.; Hunt, D.F. “Comprehensive
Phosphoprotein Analysis of Linker Histone H1 from Tetrahymena
thermophila,” Mol. Cell. Proteomics, 2006,
in press.
Dorrestein, P.C. and Kelleher, N.L., “Dissecting non-ribosomal
and polyketide biosynthetic machineries using electrospray ionization
Fourier-Transform mass spectrometry.” Natural Product
Reports, 2006, invited review in press.
Hicks, L.M.; Moffitt, M.C.; Beer, L.L.; Moore, B.S.; Kelleher,
N.L., “Structural Characterization of In Vitro and In Vivo
Intermediates on the Loading Module of Microcystin Synthetase”,
ACS Chem. Biol., 2005, in press.
Hicks, L.M., Walsh, C.T.; Kelleher, N.L.; Hilson, N., “Probing
intra vs interchain kinetic preferences of L-Thr acylation on
dimeric VibF with mass spectrometry”, Biophys. J.,
2006, 91, 000-000.
Romero-Guss, M.C.; Shoemaker, N.B.; Boyne, M.T.; Kelleher, N.L.;
Salyers, A.A., “Identification of a novel host factor necessary
for in vitro integration of the Bacteroides conjugative transposon,
CTnDOT”, in press.
Romanova, E. V.; Roth, M. J.; Rubakhin, S.S.; Jakubowski, J.A.;
Kelley, W.P.; Kirk, M. D.; Kelleher, N.L.; Sweedler, J.V., "Homologues
of Vertebrate Beta-Thymosin Expressed in the Aplysia Central Nervous
System Modulate Neurite Growth in Cultured Neurons", J.
Mass. Spectrom., 2006, accepted.
Calderone CT, Kowtoniuk WE, Kelleher NL, Walsh CT, Dorrestein
PC. Related Articles, Links Convergence of isoprene and polyketide
biosynthetic machinery: Isoprenyl-S-carrier proteins in the pksX
pathway of Bacillus subtilis.” Proc. Natl. Acad. Sci.
U.S.A., 2006, Jun. 6, Epub ahead of print.
Hicks, L.M.; Mazur, M.T.; Miller, L.M.; Dorrestein, P.C.; Schnarr,
N.A.; Khosla; C.; Kelleher, N.L., “Investigating Nonribosomal
Peptide and Polyketide Biosynthesis by Direct Detection of Intermediates
on >70 kDa Polypeptides using Fourier-Transform Mass Spectrometry”,
ChemBioChem., 2006, 7(6), 904-907.
Zhang, Z.; An, X.; Yang, K.; Perlstein, D. L.; Hicks, L.M.; Kelleher,
N.L.; Stubbe, J.; and Huang, M., “Nuclear Localization of
the Saccharomyces cerevisiae Ribonucleotide Reductase Small Subunit
Requires a Karyopherin and a WD40 Repeat Protein”, Proc.
Natl. Acad. Sci. U.S.A., 2006, 103,
1422-1427.
Howarth, M.; Chinnapen, D.; Gerrow, K.; Dorrestein, P.C; Grandy,
M.; Kelleher, N.L.; Alaa El-Husseini, A.; Ting, A. “A monovalent
streptavidin with a single femtomolar biotin binding site”,
Nat. Methods, 2006, 3, 267-273.
Gatto G.J. Jr.; Boyne, M.; Kelleher, N.L.; Walsh, C.T. “Biosynthesis
of Pipecolic Acid by RapL, a Lysine Cyclodeaminase Encoded in
the Rapamycin Gene Cluster” J. Am. Chem. Soc.,
2006, 128, 3838-3347.
Dorrestein, P.C.; Blackhall, J.; Straight, P. D., Fischbach,
M.A.; Garneau- Tsodikova, S.; Edwards, D.J.; McLaughlin, S.M.;
Lin, M.; Gerwick, W.H.; Kolter, R.; Walsh, C.T.; Kelleher, N.L.
“Activity Screening of Carrier Domains within Nonribosomal
Peptide Synthetases Using Complex Substrate Mixtures and Large
Molecule Mass Spectrometry”, Biochemistry, 2006,
45(6), 1537-1546. Published as an Accelerated Article.
Pesavento, J. J.; Mizzen, C. A.; Kelleher, N. L. “Quantitative
Analysis of Modified Proteins and Their Positional Isomers by
Tandem Mas Spectrometry: Human Histone H4.” Anal. Chem.,
Epub ahead of press.
Siuti, N.; Roth, M.; Mizzen, C. A.; Kelleher, N. L.; Pesavento,
J. J. “Gene-Specific Characterization of Human Histone H2B
by Electron Capture Dissociation.” J. Proteome Res.,
2006, 5, 233-239.
Thomas, C. E.; Mizzen, C. A.; Kelleher, N. L. “Mass Spectrometric
Characterization of Human Histone H3: A Bird's Eye View.”
J. Proteome Res., 2006, 5,
240-247.
Boyne, M. T.; Pesavento, J. J.; Mizzen, C. A.; Kelleher, N. L.
“Precise Characterization of Human Histones in the H2A Gene
Family by Top Down Mass Spectrometry.” J. Proteome Res.,
2006, 5, 248-253.
Du, Y.; Parks, B.A.; Sohn, S.; Kwast, K.E.; Kelleher, N.L. “Top
Down Approaches for Measuring Expression Ratios of Intact Yeast
Proteins using Fourier-Transform Mass Spectrometry,” Anal.
Chem., 2006, 78, 686--694.
Miller, L.M.; Chatterjee, C.; van der Donk, W.A.; Kelleher, N.L.;
“The Dehydratase Activity of Lacticin 481 Synthetase is
Highly Processive”, J. Am. Chem. Soc., 2005,
128, 1420-1421.
Yin, J.; Straight, P.D.; McLaughlin, S.M.; Zhou, Z.; Lin, A.J.;
Golan, D.E.; Kelleher, N.L.; Kolter, R.; Walsh, C.T. “Genetically
encoded short peptide tag for versatile protein labeling by Sfp
phosphopantetheinyl transferase”, Proc. Natl. Acad.
Sci. USA, 2005, 102, 15815-15820.
Chatterjee, C.; Miller, L.M.; Leung, Y.L.; Xie, L.; Yi, M.;
Kelleher, N.L.; van der Donk, W.A. "Lacticin 481 Synthetase
Phosphorylates its Substrate during Lantibiotic Production",
J. Am. Chem. Sco., 2005, 127, 15332-15333.
Patrie, S.M.; Ferguson, J.T.; Robinson, D.E.; Rother, M.; Metcalf,
W.; Kelleher, N.L., "Top Down Mass Spectrometry of <60
kDa Proteins from Methanosarcina Acetivorans by Automated
Octupole Collisionally Activated Dissociation", Mol.
Cell Proteomics, 2005, Oct 18, Epub
ahead of print.
Yin, J.; Straight, P.D.; McLaughlin, S.M.; Zhou, Z.; Lin, A.J.;
Golan, D.E.; Kelleher, N.L.; Kolter, R.; Walsh, C.T. "Genetically
encoded short peptide tag for versatile protein labeling by Sfp
phosphopantetheinyl transferase", Proc. Natl. Acad. Sci.
USA, 2005, 102, 15815-15820.
Aron, Z.D.; Dorrestein, P.C.; Blackhall, J.R.; Kelleher, N.L;
Walsh, C.L., "Characterization of a New Tailoring Domain
in Polyketide Biogenesis: The Amine Transferase Domain of MycA
in the Mycosubtilin Gene Cluster", J. Am. Chem. Soc.,
2005, 127, 14986-14987.
McLoughlin, S. M. and Kelleher, N. L., " Monitoring Multiple
Active Sites on Thiotemplate Enzymes in Parallel: A Molecular
Movie of Yersiniabactin Bioassembly", J. Am. Chem. Soc.,
2005, 127, 14984--14985.
Kelleher, N.L. and Hicks, L. M., "Contemporary Mass Spectrometry
for Direct Detection of Enzyme Intermediates", Invited
Review for Critical Rev. Chem. Biol. (guest Editors, Rowena
Matthews and Chris Walsh), 2005, 9, 424-430.
Zubrouskov, V.; Senko, M.W.; Du, Y.; Leduc, R.D.; Kelleher, N.L.,
“New and Automated MS(n) Approaches for Top-Down Identification
of Modified Proteins,” J. Am. Soc. Mass Spectrom.,
2005, 16, 2027-2038.
McLoughlin, S. M.; Mazur, M. T.; Miller, L. M.; Yin, J.; Liu,
F.; Walsh, C. T.; Kelleher, N. L., "Chemoenzymatic Approaches
for Streamlined Detection of Active Site Modifications on Thiotemplate
Assembly Lines Using Mass Spectrometry", Biochemistry,
2005, 44, 14159-14169.
Dorrestein PC, Yeh E, Garneau-Tsodikova S, Kelleher NL, Walsh
CT “Dichlorination of a pyrrolyl-S-carrier protein by FADH2-dependent
halogenase PltA during pyoluteorin biosynthesis”, Proc.
Natl. Acad. Sci. USA, 2005, 102,
13843-13848.
Van Lanen, S.G.; Dorrestein, P.C.; Christenson, S.D.; Liu, W.;
Ju, J.; Kelleher, N.L.; Shen, B. “Biosynthesis of the beta-amino
acid moiety of the enediyne antitumor antibiotic C-1027 featuring
beta-amino acyl-S-carrier protein intermediates”, J.
Am. Chem. Soc., 2005, 127, 11594-11595.
Frankel; B.A.; Kruger, R.G.; Robinson, D.E.; Kelleher, N.L.; McCafferty,
D.G., "Staphylococcus aureus sortase transpeptidase
SrtA: Insight into the kinetic mechanism and evidence for a reverse
protonation catalytic mechanism", Biochemistry, 2005,
44, 11188-11200.
Roth, M. J.; Forbes, A. J.; Boyne, M. T.; Robinson, D. E.; Kelleher,
N. L. , "Precise Characterization of Coding Polymorphisms,
Alternative Splice Variants, and Modifications in Human Proteins
by Top Down Mass Spectrometry", Mol. Cell. Proteomics,
2005, 4, 1002-1008.
Miller, L. M.; Mazur, M.T.; McLoughlin, S.M.; Kelleher, N. L.,
"Parallel interrogation of covalent intermediates in the
biosynthesis of gramicidin S using high resolution mass spectrometry",
Protein Science, 2005, 14, 2702-2712.
Gatto G.J. Jr; McLoughlin, S.M.; Kelleher ,N.L.; Walsh C.T., "Elucidating
the Substrate Specificity and Condensation Domain Activity of
FkbP, the FK520 Pipecolate-Incorporating Enzyme", Biochemistry,
2005, 44, 5993-6002.
Garneau S, ; Dorrestein, P.C.; Kelleher, N.L.; Walsh, C.T. , "Characterization
of the formation of the pyrrole moiety during clorobiocin and
coumermycin A1 biosynthesis", Biochemistry, 2005,
44, 2770-80.
Meng, F.; Forbes, A. J.; Miller, L.M.; Kelleher, N.L. "Detection
and Localization of Protein Modifications by High-Resolution Tandem
Mass Spectrometry", Mass Spectrom. Rev., 2005,
24, 126-134.
McLoughlin, S. and Kelleher, N. L. "Kinetic and Regiospecific
Interrogation of Covalent Intermediates in the Biosynthesis of
Yersiniabactin", J. Am. Chem. Soc., 2004,
126, 13265-13275.
Du, Y.; Meng, F.; Patrie, S.M.; Miller, L.M.; Kelleher, N.L. "Improved
molecular weight-based processing of intact proteins for interrogation
by quadrupole-enhanced FT MS/MS" ,J. Proteome Res.,
2004, 3, 801-806.
Pesavento, J.P.; Taylor, G.K.; Kim, Y.B.; Kelleher, N.L. "Shotgun
Annotation of Histone Modifications: A New Approach for Streamlined
Characterization of Proteins by Top Down Mass Spectrometry",
J. Am. Chem. Soc., 2004, 126,
3386-3387.
Leduc, R.; Taylor, G.K.; Kim, Y.B.; Januszyk, T.; Bynum, L.; Sola,
J.; Garavelli, J. S.; Kelleher, N.L., "ProSight PTM: an integrated
environment for protein identification and characterization by
top-down mass spectrometry", Nucleic Acids Res.,
2004, 32, W340-W345.
Meng, F.; Du, Y.; Miller, L.M.; Patrie, S.M.; Robinson, D.E.;
Taylor, G.K.; Kim, Y.B.; Kelleher, N.L., "Molecular-Level
Description of Proteins from Saccharomyces cerevisiae
using Q-FT Mass Spectrometry for Top Down Proteomics", Anal.
Chem., 2004, 76, 2852-2858.
Patrie, S.M.; Robinson, D.E.; Meng, F.; Kelleher, N.L., "Strategies
for Automating Top Down Proteomics with Q-FT ICR MS",Int.
J. Mass Spectrom., 2004, 234, 175-184
.
Forbes, A.J.; Patrie, S.M.; Taylor, G.K.; Kim, Y.B.; Kelleher,
N.L., "Targeted Analysis and Discovery of Post-Translational
Modifications in Proteins from Methanogenic Archaea by Top Down
Mass Spectrometry, Proc. Natl. Acad. Sci USA, 2004,
101 (9), 2678-2683.
Patrie, S.M.; Charlebois, J.P.; Whipple D, Hendrickson CL, Quinn
JP, Marshall AG, Mukhopadhyay B. Kelleher, N.L., "Construction
of a Hybrid Quadrupole/Fourier-Transform Mass Spectrometer for
Versatile MS/MS Above 10 kDa", J. Am. Soc. Mass Spectrom.,
2004, 15, 1099-1108.
Kelleher, N.L., "Top Down Proteomics", Anal. Chem.,
2004, 76, 197A-203A. (A Peer-reviewed
perspective.)
Xie, L.; Miller, L. M.; Averin, O.; Chatterjee, C.; Kelleher,
N.L.; van der Donk, W. A., "Lacticin 481: in vitro
reconstitution of lantibiotic synthetase activity", Science,
2004, 303, 679-681.
Tseng, C.C.; McLoughlin, S.M.; Kelleher, N.L.; Walsh, C.T., "Role
of the active site cysteine of DpgA, a bacterial type III polyketide
synthase", Biochemistry, 2004,
43, 970-980.
Hicks, L.M; O'Connor, S.E.; Mazur, M.T.; Walsh, C.T.; Kelleher,
N.L., "Mass spectrometric interrogation of thioester-bound
intermediates in the initial stages of epothilone biosynthesis",
Chem. & Biol., 2004, 11,
327-335. (Featured on the Cover).
Mazur, M.T.; Walsh, C.T.; Kelleher, N.L., "Site-Specific
Observation of Acyl-Intermediate Processing in Thiotemplate Biosynthesis
by Fourier-Transform Mass Spectrometry: The Polyketide Module
of Yersiniabactin Synthetase", Biochemistry, 2003,
42, 13393-13400 (Printed as an Accelerated Article).
He, S.; Bauman, D.; Davis, J.S.; Loyola, A.; Nishioka, K.; Gronlund,
J.; Reinberg, D.; Meng, F.; Kelleher, N. L.; McCafferty, D.G.,
"Facile synthesis of site-specifically acetylated and methylated
histone proteins: reagents for evaluation of the histone code
hypothesis", Proc. Natl. Acad. Sci. USA, 2003,
100, 12033-12038.
Taylor, G. K.; Kim, Y-B; Forbes, A.J.; Meng, F.; McCarthy, R;
Kelleher, N.L. "Web and Database Software for Analysis of
Intact Proteins By Top Down Mass Spectrometry," Anal.
Chem., 2003, 75, 4081-4086.
Charlebois, J. P.; Patrie, S. M.; Kelleher, N. L., "Electron
Capture Dissociation and 13C/15N Depletion
for Improved Deuterium Localization for Intact Proteins after
Solution Phase Exchange", Anal. Chem., 2003,
75, 3263-3266.
Hicks, L.M.; Weinreb, P. H.; Konz, D.; Marahiel, M.; Walsh, C.
T. ; Kelleher, N. L., "FT Mass Spectrometry for Detection
of Thioester-Bound Intermediates in Unfractionated Proteolytic
Mixtures of 80 and 191 kDa Portions of Bacitracin A Synthetase",
Anal. Chim. Acta, 2003, 496,
217-224. (Special Issue for North American Young Faculty.)
Johnson, J.R. ; Cargile, B. J. ;Meng, F. ; Forbes, A. J. ; Kelleher,
N. L. ,"FT Mass Spectrometry for Automated Fragmentation
and Identification of 5-20 Proteins in Mixtures", Electrophoresis,
2002, 23 (18), 3217-3223.
Meng, F.; Cargile, B. J.; Patrie, S. M.; Johnson, J. R.; McLoughlin,
S. M.; Kelleher, N. L. "Processing Complex Mixtures of Intact
Proteins for Direct Analysis by Mass Spectrometry", Anal.
Chem., 2002, 74, 2923-2929.
Valaskovic,G. A. and Kelleher,N. L. "Miniaturized Formats
for Efficient Mass Spectrometry-Based Proteomics and Therapeutic
Development", Curr. Topics Med. Chem. (Review),
2002, 2 (1), 1-12.
Meng, F. ; Cargile, B. J. ; Miller, L.M.; Johnson, J. R. ; Kelleher,
N. L. , "Informatics and Multiplexing of Intact Protein Identification
in Bacteria and The Archaea", Nat. Biotechnol.,
2001, 19, 952-956.
Forbes, A. J.;Mazur, M. ; Patel, H. M.; Walsh, C. T.; Kelleher,
N.L., "Toward Efficient Analysis of >70 kDa Proteins with
100% Sequence Coverage,@ Proteomics, 2001,
1 (8), 927-933 and Eur. J. Mass Spectrom. (a
joint issue), 2001, 7, 81-87.
N. L. Kelleher, "A Newcomer Ponders the Chemical Sciences",
Chem. & Eng. News, 2001, March
26 (Issue Celebrating The ACS on its 125th Anniversary),
252.
Kelleher, N. L. "From Primary Structure to Function: Biological
Insights from Large Molecule Mass Spectra", Chem. &
Biol., 2000, 7, R37-R45.
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