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 :: UIUC :: School of Chemical Sciences :: Chemistry :: Kelleher Group :: Group Members :: Neil Kelleher ::

Assistant Professor Kelleher with the Illinois 8.5T Q-FTMS
Neil L. Kelleher
Associate Professor of Chemistry
with the Illinois 8.5T Q-FTMS
 

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Neil L. Kelleher

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Education

  • Cornell University, Ithaca, NY
  • Ph.D. in Bioanalytical Chemistry, August, 1997
  • M.S. in Bioanalytical Chemistry, 1995
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  • University of Konstanz, Germany, Chemistry Dept.
  • Organic Synthesis, 1992 - 1993
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  • Pacific Lutheran University (PLU), Tacoma, WA
  • B. S. in Chemistry and B. A. in German, 1992

Professional Organizations

  • American Chemical Society (ACS)
  • American Society of Mass Spectrometry (ASMS)
  • Am. Assoc. for the Advan. of Science (AAAS)

Honors & Awards

Professional Experience

  • Associate Professor, University of Illinois at Urbana-Champaign (7/99 - present)
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  • Postdoctoral Associate, Harvard Medical School (11/97 - 6/99):
  • Research with Prof. Christopher Walsh focused on mechanistic studies of enzymes involved in the biosynthesis of peptide antibiotics.
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  • Research Assistant, Cornell University (8/93 - 11/97):
  • With Professors T. P. Begley and F. W. McLafferty, electrospray ionization Fourier-transform mass spectrometry (ESI/FTMS) was used to study the structure and function of bacterial enzymes in thiamin biosynthesis. Assisted in training of numerous group members and undergraduates.
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  • Fulbright Scholar, University of Konstanz (9/92 - 7/93):
  • Research under Prof. Wolfgang Pfleiderer focused on an improved synthesis for tetrahydrobiopterin, a cofactor for phenylalanine-4-hydroxylase, and its analogs.

Publications

    Pesavento, J.P.; Kelleher, N.L.; Mizzen C.A. “Histone H4 from Normal and Cancer Cells.” Nature Genetics, under review.

    Garcia, B.A.; Siuti, N.; Thomas, C.E.; Mizzen, C.A.; Kelleher, N.L. “Characterization of Neurohistone Variants and Post-translational Modifications by Electron Capture Dissociation Mass Spectrometry,” Int. J. Mass Spectrom., 2006, accepted. (Special issue honoring Donald F. Hunt on his 65th Birthday.)

    Garcia, B.A.; Joshi, S.; Thomas, C.E.; Chitta, R.K.; Diaz, R.L.; Busby, S.A.; Andrews, P.C.; Ogorzalek Loo, R.R.; Shabanowitz, J.; Kelleher, N.L.; Mizzen, C.A.; Allis, C.D.; Hunt, D.F. “Comprehensive Phosphoprotein Analysis of Linker Histone H1 from Tetrahymena thermophila,” Mol. Cell. Proteomics, 2006, in press.

    Dorrestein, P.C. and Kelleher, N.L., “Dissecting non-ribosomal and polyketide biosynthetic machineries using electrospray ionization Fourier-Transform mass spectrometry.” Natural Product Reports, 2006, invited review in press.

    Hicks, L.M.; Moffitt, M.C.; Beer, L.L.; Moore, B.S.; Kelleher, N.L., “Structural Characterization of In Vitro and In Vivo Intermediates on the Loading Module of Microcystin Synthetase”, ACS Chem. Biol., 2005, in press.

    Hicks, L.M., Walsh, C.T.; Kelleher, N.L.; Hilson, N., “Probing intra vs interchain kinetic preferences of L-Thr acylation on dimeric VibF with mass spectrometry”, Biophys. J., 2006, 91, 000-000.

    Romero-Guss, M.C.; Shoemaker, N.B.; Boyne, M.T.; Kelleher, N.L.; Salyers, A.A., “Identification of a novel host factor necessary for in vitro integration of the Bacteroides conjugative transposon, CTnDOT”, in press.

    Romanova, E. V.; Roth, M. J.; Rubakhin, S.S.; Jakubowski, J.A.; Kelley, W.P.; Kirk, M. D.; Kelleher, N.L.; Sweedler, J.V., "Homologues of Vertebrate Beta-Thymosin Expressed in the Aplysia Central Nervous System Modulate Neurite Growth in Cultured Neurons", J. Mass. Spectrom., 2006, accepted.

    Calderone CT, Kowtoniuk WE, Kelleher NL, Walsh CT, Dorrestein PC. Related Articles, Links Convergence of isoprene and polyketide biosynthetic machinery: Isoprenyl-S-carrier proteins in the pksX pathway of Bacillus subtilis.” Proc. Natl. Acad. Sci. U.S.A., 2006, Jun. 6, Epub ahead of print.

    Hicks, L.M.; Mazur, M.T.; Miller, L.M.; Dorrestein, P.C.; Schnarr, N.A.; Khosla; C.; Kelleher, N.L., “Investigating Nonribosomal Peptide and Polyketide Biosynthesis by Direct Detection of Intermediates on >70 kDa Polypeptides using Fourier-Transform Mass Spectrometry”, ChemBioChem., 2006, 7(6), 904-907.

    Zhang, Z.; An, X.; Yang, K.; Perlstein, D. L.; Hicks, L.M.; Kelleher, N.L.; Stubbe, J.; and Huang, M., “Nuclear Localization of the Saccharomyces cerevisiae Ribonucleotide Reductase Small Subunit Requires a Karyopherin and a WD40 Repeat Protein”, Proc. Natl. Acad. Sci. U.S.A., 2006, 103, 1422-1427.

    Howarth, M.; Chinnapen, D.; Gerrow, K.; Dorrestein, P.C; Grandy, M.; Kelleher, N.L.; Alaa El-Husseini, A.; Ting, A. “A monovalent streptavidin with a single femtomolar biotin binding site”, Nat. Methods, 2006, 3, 267-273.

    Gatto G.J. Jr.; Boyne, M.; Kelleher, N.L.; Walsh, C.T. “Biosynthesis of Pipecolic Acid by RapL, a Lysine Cyclodeaminase Encoded in the Rapamycin Gene Cluster” J. Am. Chem. Soc., 2006, 128, 3838-3347.

    Dorrestein, P.C.; Blackhall, J.; Straight, P. D., Fischbach, M.A.; Garneau- Tsodikova, S.; Edwards, D.J.; McLaughlin, S.M.; Lin, M.; Gerwick, W.H.; Kolter, R.; Walsh, C.T.; Kelleher, N.L. “Activity Screening of Carrier Domains within Nonribosomal Peptide Synthetases Using Complex Substrate Mixtures and Large Molecule Mass Spectrometry”, Biochemistry, 2006, 45(6), 1537-1546. Published as an Accelerated Article.

    Pesavento, J. J.; Mizzen, C. A.; Kelleher, N. L. “Quantitative Analysis of Modified Proteins and Their Positional Isomers by Tandem Mas Spectrometry: Human Histone H4.” Anal. Chem., Epub ahead of press.

    Siuti, N.; Roth, M.; Mizzen, C. A.; Kelleher, N. L.; Pesavento, J. J. “Gene-Specific Characterization of Human Histone H2B by Electron Capture Dissociation.” J. Proteome Res., 2006, 5, 233-239.

    Thomas, C. E.; Mizzen, C. A.; Kelleher, N. L. “Mass Spectrometric Characterization of Human Histone H3: A Bird's Eye View.” J. Proteome Res., 2006, 5, 240-247.

    Boyne, M. T.; Pesavento, J. J.; Mizzen, C. A.; Kelleher, N. L. “Precise Characterization of Human Histones in the H2A Gene Family by Top Down Mass Spectrometry.” J. Proteome Res., 2006, 5, 248-253.

    Du, Y.; Parks, B.A.; Sohn, S.; Kwast, K.E.; Kelleher, N.L. “Top Down Approaches for Measuring Expression Ratios of Intact Yeast Proteins using Fourier-Transform Mass Spectrometry,” Anal. Chem., 2006, 78, 686--694.

    Miller, L.M.; Chatterjee, C.; van der Donk, W.A.; Kelleher, N.L.; “The Dehydratase Activity of Lacticin 481 Synthetase is Highly Processive”, J. Am. Chem. Soc., 2005, 128, 1420-1421.

    Yin, J.; Straight, P.D.; McLaughlin, S.M.; Zhou, Z.; Lin, A.J.; Golan, D.E.; Kelleher, N.L.; Kolter, R.; Walsh, C.T. “Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase”, Proc. Natl. Acad. Sci. USA, 2005, 102, 15815-15820.

    Chatterjee, C.; Miller, L.M.; Leung, Y.L.; Xie, L.; Yi, M.; Kelleher, N.L.; van der Donk, W.A. "Lacticin 481 Synthetase Phosphorylates its Substrate during Lantibiotic Production", J. Am. Chem. Sco., 2005, 127, 15332-15333.

  • Patrie, S.M.; Ferguson, J.T.; Robinson, D.E.; Rother, M.; Metcalf, W.; Kelleher, N.L., "Top Down Mass Spectrometry of <60 kDa Proteins from Methanosarcina Acetivorans by Automated Octupole Collisionally Activated Dissociation", Mol. Cell Proteomics, 2005, Oct 18, Epub ahead of print.
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  • Yin, J.; Straight, P.D.; McLaughlin, S.M.; Zhou, Z.; Lin, A.J.; Golan, D.E.; Kelleher, N.L.; Kolter, R.; Walsh, C.T. "Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase", Proc. Natl. Acad. Sci. USA, 2005, 102, 15815-15820.
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  • Aron, Z.D.; Dorrestein, P.C.; Blackhall, J.R.; Kelleher, N.L; Walsh, C.L., "Characterization of a New Tailoring Domain in Polyketide Biogenesis: The Amine Transferase Domain of MycA in the Mycosubtilin Gene Cluster", J. Am. Chem. Soc., 2005, 127, 14986-14987.

    McLoughlin, S. M. and Kelleher, N. L., " Monitoring Multiple Active Sites on Thiotemplate Enzymes in Parallel: A Molecular Movie of Yersiniabactin Bioassembly", J. Am. Chem. Soc., 2005, 127, 14984--14985.

    Kelleher, N.L. and Hicks, L. M., "Contemporary Mass Spectrometry for Direct Detection of Enzyme Intermediates", Invited Review for Critical Rev. Chem. Biol. (guest Editors, Rowena Matthews and Chris Walsh), 2005, 9, 424-430.  

  • Zubrouskov, V.; Senko, M.W.; Du, Y.; Leduc, R.D.; Kelleher, N.L., “New and Automated MS(n) Approaches for Top-Down Identification of Modified Proteins,” J. Am. Soc. Mass Spectrom., 2005, 16, 2027-2038.

    McLoughlin, S. M.; Mazur, M. T.; Miller, L. M.; Yin, J.; Liu, F.; Walsh, C. T.; Kelleher, N. L., "Chemoenzymatic Approaches for Streamlined Detection of Active Site Modifications on Thiotemplate Assembly Lines Using Mass Spectrometry", Biochemistry, 2005, 44, 14159-14169.

    Dorrestein PC, Yeh E, Garneau-Tsodikova S, Kelleher NL, Walsh CT “Dichlorination of a pyrrolyl-S-carrier protein by FADH2-dependent halogenase PltA during pyoluteorin biosynthesis”, Proc. Natl. Acad. Sci. USA, 2005, 102, 13843-13848.

    Van Lanen, S.G.; Dorrestein, P.C.; Christenson, S.D.; Liu, W.; Ju, J.; Kelleher, N.L.; Shen, B. “Biosynthesis of the beta-amino acid moiety of the enediyne antitumor antibiotic C-1027 featuring beta-amino acyl-S-carrier protein intermediates”, J. Am. Chem. Soc., 2005, 127, 11594-11595. 

  • Frankel; B.A.; Kruger, R.G.; Robinson, D.E.; Kelleher, N.L.; McCafferty, D.G., "Staphylococcus aureus sortase transpeptidase SrtA: Insight into the kinetic mechanism and evidence for a reverse protonation catalytic mechanism", Biochemistry, 2005, 44, 11188-11200.
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  • Roth, M. J.; Forbes, A. J.; Boyne, M. T.; Robinson, D. E.; Kelleher, N. L. , "Precise Characterization of Coding Polymorphisms, Alternative Splice Variants, and Modifications in Human Proteins by Top Down Mass Spectrometry", Mol. Cell. Proteomics, 2005, 4, 1002-1008.

    Miller, L. M.; Mazur, M.T.; McLoughlin, S.M.; Kelleher, N. L., "Parallel interrogation of covalent intermediates in the biosynthesis of gramicidin S using high resolution mass spectrometry", Protein Science, 2005, 14, 2702-2712.
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  • Gatto G.J. Jr; McLoughlin, S.M.; Kelleher ,N.L.; Walsh C.T., "Elucidating the Substrate Specificity and Condensation Domain Activity of FkbP, the FK520 Pipecolate-Incorporating Enzyme", Biochemistry, 2005, 44, 5993-6002.
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  • Garneau S, ; Dorrestein, P.C.; Kelleher, N.L.; Walsh, C.T. , "Characterization of the formation of the pyrrole moiety during clorobiocin and coumermycin A1 biosynthesis", Biochemistry, 2005, 44, 2770-80.
  • Meng, F.; Forbes, A. J.; Miller, L.M.; Kelleher, N.L. "Detection and Localization of Protein Modifications by High-Resolution Tandem Mass Spectrometry", Mass Spectrom. Rev., 2005, 24, 126-134.
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  • McLoughlin, S. and Kelleher, N. L. "Kinetic and Regiospecific Interrogation of Covalent Intermediates in the Biosynthesis of Yersiniabactin", J. Am. Chem. Soc., 2004, 126, 13265-13275.
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  • Du, Y.; Meng, F.; Patrie, S.M.; Miller, L.M.; Kelleher, N.L. "Improved molecular weight-based processing of intact proteins for interrogation by quadrupole-enhanced FT MS/MS" ,J. Proteome Res., 2004, 3, 801-806.
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  • Pesavento, J.P.; Taylor, G.K.; Kim, Y.B.; Kelleher, N.L. "Shotgun Annotation of Histone Modifications: A New Approach for Streamlined Characterization of Proteins by Top Down Mass Spectrometry", J. Am. Chem. Soc., 2004, 126, 3386-3387.
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  • Leduc, R.; Taylor, G.K.; Kim, Y.B.; Januszyk, T.; Bynum, L.; Sola, J.; Garavelli, J. S.; Kelleher, N.L., "ProSight PTM: an integrated environment for protein identification and characterization by top-down mass spectrometry", Nucleic Acids Res., 2004, 32, W340-W345.
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  • Meng, F.; Du, Y.; Miller, L.M.; Patrie, S.M.; Robinson, D.E.; Taylor, G.K.; Kim, Y.B.; Kelleher, N.L., "Molecular-Level Description of Proteins from Saccharomyces cerevisiae using Q-FT Mass Spectrometry for Top Down Proteomics", Anal. Chem., 2004, 76, 2852-2858.
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  • Patrie, S.M.; Robinson, D.E.; Meng, F.; Kelleher, N.L., "Strategies for Automating Top Down Proteomics with Q-FT ICR MS",Int. J. Mass Spectrom., 2004, 234, 175-184 .
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  • Forbes, A.J.; Patrie, S.M.; Taylor, G.K.; Kim, Y.B.; Kelleher, N.L., "Targeted Analysis and Discovery of Post-Translational Modifications in Proteins from Methanogenic Archaea by Top Down Mass Spectrometry, Proc. Natl. Acad. Sci USA, 2004, 101 (9), 2678-2683.
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  • Patrie, S.M.; Charlebois, J.P.; Whipple D, Hendrickson CL, Quinn JP, Marshall AG, Mukhopadhyay B. Kelleher, N.L., "Construction of a Hybrid Quadrupole/Fourier-Transform Mass Spectrometer for Versatile MS/MS Above 10 kDa", J. Am. Soc. Mass Spectrom., 2004, 15, 1099-1108.
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  • Kelleher, N.L., "Top Down Proteomics", Anal. Chem., 2004, 76, 197A-203A. (A Peer-reviewed perspective.)
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  • Xie, L.; Miller, L. M.; Averin, O.; Chatterjee, C.; Kelleher, N.L.; van der Donk, W. A., "Lacticin 481: in vitro reconstitution of lantibiotic synthetase activity", Science, 2004, 303, 679-681.
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  • Tseng, C.C.; McLoughlin, S.M.; Kelleher, N.L.; Walsh, C.T., "Role of the active site cysteine of DpgA, a bacterial type III polyketide synthase", Biochemistry, 2004, 43, 970-980.
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  • Hicks, L.M; O'Connor, S.E.; Mazur, M.T.; Walsh, C.T.; Kelleher, N.L., "Mass spectrometric interrogation of thioester-bound intermediates in the initial stages of epothilone biosynthesis", Chem. & Biol., 2004, 11, 327-335. (Featured on the Cover).
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  • Mazur, M.T.; Walsh, C.T.; Kelleher, N.L., "Site-Specific Observation of Acyl-Intermediate Processing in Thiotemplate Biosynthesis by Fourier-Transform Mass Spectrometry: The Polyketide Module of Yersiniabactin Synthetase", Biochemistry, 2003, 42, 13393-13400 (Printed as an Accelerated Article).
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  • He, S.; Bauman, D.; Davis, J.S.; Loyola, A.; Nishioka, K.; Gronlund, J.; Reinberg, D.; Meng, F.; Kelleher, N. L.; McCafferty, D.G., "Facile synthesis of site-specifically acetylated and methylated histone proteins: reagents for evaluation of the histone code hypothesis", Proc. Natl. Acad. Sci. USA, 2003, 100, 12033-12038.
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  • Taylor, G. K.; Kim, Y-B; Forbes, A.J.; Meng, F.; McCarthy, R; Kelleher, N.L. "Web and Database Software for Analysis of Intact Proteins By Top Down Mass Spectrometry," Anal. Chem., 2003, 75, 4081-4086.
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  • Charlebois, J. P.; Patrie, S. M.; Kelleher, N. L., "Electron Capture Dissociation and 13C/15N Depletion for Improved Deuterium Localization for Intact Proteins after Solution Phase Exchange", Anal. Chem., 2003, 75, 3263-3266.
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  • Hicks, L.M.; Weinreb, P. H.; Konz, D.; Marahiel, M.; Walsh, C. T. ; Kelleher, N. L., "FT Mass Spectrometry for Detection of Thioester-Bound Intermediates in Unfractionated Proteolytic Mixtures of 80 and 191 kDa Portions of Bacitracin A Synthetase", Anal. Chim. Acta, 2003, 496, 217-224. (Special Issue for North American Young Faculty.)
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  • Johnson, J.R. ; Cargile, B. J. ;Meng, F. ; Forbes, A. J. ; Kelleher, N. L. ,"FT Mass Spectrometry for Automated Fragmentation and Identification of 5-20 Proteins in Mixtures", Electrophoresis, 2002, 23 (18), 3217-3223.
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  • Meng, F.; Cargile, B. J.; Patrie, S. M.; Johnson, J. R.; McLoughlin, S. M.; Kelleher, N. L. "Processing Complex Mixtures of Intact Proteins for Direct Analysis by Mass Spectrometry", Anal. Chem., 2002, 74, 2923-2929.
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  • Valaskovic,G. A. and Kelleher,N. L. "Miniaturized Formats for Efficient Mass Spectrometry-Based Proteomics and Therapeutic Development", Curr. Topics Med. Chem. (Review), 2002, 2 (1), 1-12.
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  • Meng, F. ; Cargile, B. J. ; Miller, L.M.; Johnson, J. R. ; Kelleher, N. L. , "Informatics and Multiplexing of Intact Protein Identification in Bacteria and The Archaea", Nat. Biotechnol., 2001, 19, 952-956.
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  • Forbes, A. J.;Mazur, M. ; Patel, H. M.; Walsh, C. T.; Kelleher, N.L., "Toward Efficient Analysis of >70 kDa Proteins with 100% Sequence Coverage,@ Proteomics, 2001, 1 (8), 927-933 and Eur. J. Mass Spectrom. (a joint issue), 2001, 7, 81-87.
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  • N. L. Kelleher, "A Newcomer Ponders the Chemical Sciences", Chem. & Eng. News, 2001, March 26 (Issue Celebrating The ACS on its 125th Anniversary), 252.
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  • Kelleher, N. L. "From Primary Structure to Function: Biological Insights from Large Molecule Mass Spectra", Chem. & Biol., 2000, 7, R37-R45.
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  • Roman A. Zubarev, David M. Horn, Einar K. Fridriksson, Neil L. Kelleher, Nathan A. Kruger, Mark A. Lewis, Barry K. Carpenter, and Fred W. McLafferty, Electron Capture Dissociation for Structural Characterization of Multiply Charged Protein Cations, Anal. Chem., 2000, 72, 563-573.
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  • N. L. Kelleher, R. A. Zubarev, K. Bush, B. Furie, B. C. Furie, F. W. McLafferty, and C. T. Walsh, “Localization of Labile Posttranslational Modifications by Electron Capture Dissociation: The Case of _-Carboxyglutamic Acid,” Anal. Chem., 1999, 71, 4250-4253.
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  • R. A. Zubarev, E. K. Fridriksson, D. M. Horn, N. L. Kelleher, N. A. Kruger, M. A. Lewis, B. K. Carpenter; F. W. McLafferty, "Electron Capture Dissociations Produces Many More Protein Backbone Cleavages Than Collisional and IR Excitation," in Mass Spectrometry in Biology and Medicine; Burlingame, A. L., Carr, S. A., and Baldwin, M. A., Eds.; 1999, pp 111-120.
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  • N. L. Kelleher, C. L. Hendrickson, and C. T. Walsh, "Posttranslational Heterocyclization of Cysteine and Serine Residues in the Antibiotic Microcin B17: Distributivity and Directionality", Biochemistry, 1999, 38, 15623-15630.
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  • N. A. Kruger, R. A. Zubarev, B. K. Carpenter, N. L. Kelleher, D. M. Horn, and F. W. McLafferty, "Electron Capture versus Energetic Dissociation of Protein Ions", Int. J. Mass Spectrom. Ion Processes, 1999, 182/183, 1-5.
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  • C. A. Shaw-Reid, N. L. Kelleher, H. C. Losey, A. G. Gehring, C. Berg, and C. T. Walsh, "Assembly Line Enzymology by Multimodular Nonribosomal Peptide Synthetases: Catalysis of Both Elongation and Cyclolactonization by the Thioesterase Domain of E. coli EntF", Chem. Biol., 1999, 6, 385-400.
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  • R. Sinha Roy, N. L. Kelleher, J. C. Milne, and C. T. Walsh, "In vivo Processing and Antibiotic Activity of Microcin B17 Variants Containing Altered Bisheterocyclic Sites", Chem. Biol., 1999, 6, 305-318.
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  • J. C. Milne, R. Sinha Roy, A. C. Eliot, N. L. Kelleher, A. Wokhlu, B. Nickels, and C. T. Walsh, "Cofactor Requirements and Reconstitution of Microcin B17 Synthetase: A Multienzyme Complex that Catalyzes the Formation of Oxazoles and Thiazoles in the Antibiotic Microcin B17", Biochemistry, 1999, 38, 4768-4781.
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  • N. L. Kelleher, H. Y. Lin, G. A. Valaskovic, D. J. Aaseruud, E. K. Fridriksson, and F. W. McLafferty, "Top Down versus Bottom Up Protein Characterization by Tandem High-Resolution Mass Spectrometry", J. Am. Chem. Soc., 1999, 121, 806-812.
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  • N. L. Kelleher, P. J. Belshaw, and C. T. Walsh, "Regioselectivity and Chemoselectivity Analysis of Oxazole and Thiazole Ring Formation by the Peptide-Heterocyclizing Microcin B17 Synthetase Using High-Resolution MS/MS", J. Am. Chem. Soc., 1998, 120, 9716-9717.
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  • F. W. McLafferty, N. L. Kelleher, T. P. Begley, E. K. Fridriksson, R. A. Zubarev, D. M. Horn, "Two-Dimensional Mass Spectrometry of Biomolecules at The Subfemtomole Level", Curr. Opin. Chem. Biol., 1998, 2, 571-578.
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  • J. C. Milne, A. E. Elliot, N. L. Kelleher, and C. T. Walsh, "ATP/GTP Hydrolysis is Required for Oxazole and Thiazole Biosynthesis in the Peptide Antibiotic Microcin B17", Biochemistry, 1998, 37, 13250-13261.
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  • P. J. Belshaw, R. Sinha Roy, N. L. Kelleher, and C. T. Walsh, "Kinetics and Regioselectivity of Peptide-to-Heterocycle Conversions by Microcin B17 Synthetase", Chem. Biol., 1998, 5, 373-384.
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  • N. L. Kelleher, S. V. Taylor, D. Grannis, H. Chiu, C. L. Kinsland, T. P. Begley, and F. W. McLafferty, "Efficient Sequence Analysis of The Six Gene Products (7-74 kDa) from the Escherichia coli Thiamin Biosynthetic Operon by High-resolution Mass Spectrometry", Prot. Sci., 1998, 7, 1796-1801.
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  • C. L. Kinsland, S. V. Taylor, N. L. Kelleher, F. W. McLafferty, and T. P. Begley, "Overexpression of Recombinant Proteins with a C-terminal Thiocarboxylate: Implications for Protein Semisynthesis and Thiamin Biosynthesis", Prot. Sci., 1998, 7, 1839-1842.
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  • S. V. Taylor, N. L. Kelleher, C. Kinsland, H. Chiu, C. A. Costello, A. D. Backstrom, F. W. McLafferty, and T. P. Begley, "Thiamin Biosynthesis in Escherichia coli. Identification of ThiS Thiocarboxylate as the Immediate Sulfur Donor In the Thiazole Formation", J. Biol. Chem., 1998, 273, 16555-16560.
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  • R. A. Zubarev, N. L. Kelleher, and F. W. McLafferty, "Electron Capture Dissociation of Multiply Charged Protein Cations. A Nonergodic Process", J. Am. Chem. Soc., 1998, 120, 3265-3266.
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  • F. W. McLafferty, Z. Guan, U. Haupts, T. D. Wood, and N. L. Kelleher, "Gaseous Conformational Structures of Cytochrome c", J. Am. Chem. Soc., 1998, 120, 4732-4740.
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  • F. W. McLafferty, D. J. Aaseruud, Z. Guan, D. P. Little, and N. L. Kelleher, "Double Stranded DNA Sequencing by Tandem Mass Spectrometry", Int. J. Mass Spectrom. Ion Processes, 1997, 165/166, 457-466.
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  • N. L. Kelleher, R. B. Nicewonger, T. P. Begley, and F. W. McLafferty, "Identification of Modification Sites in Large Biomolecules by Stable Isotope Labeling and Tandem High Resolution Mass Spectrometry: The Active Site Nucleophile of Thiaminase I", J. Biol. Chem., 1997, 272, 32215-32220.
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  • N. L. Kelleher, M. W. Senko, M. M. Siegel, and F. W. McLafferty, "Unit Resolution Mass Spectra of 112 kDa Molecules with 3 Da Accuracy", J. Am. Soc. Mass Spectrom., 1997, 8, 380-383.
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  • Z. Guan, N. L. Kelleher, P. B. O'Connor, D. J. Aaserud, D. P. Little, and F. W. McLafferty, 193 nm Photodissociation of Larger Multiply-charged Biomolecules", Int. J. Mass Spectrom. Ion Processes, 1996, 157/158, 357-364.
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  • D. J. Aaseruud, N. L. Kelleher, D. P. Little, and F. W. McLafferty, "Accurate Base Composition of Double-Strand DNA by Mass Spectrometry", J. Am. Soc. Mass Spectrom., 1996, 7, 1266-1269.
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  • G. A. Valaskovic, N. L. Kelleher, F. W. McLafferty, "Attomole Protein Characterization by Capillary Electrophoresis-Mass Spectrometry", Science, 1996, 273, 1199-1202.
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  • E. Mørtz, P. B. O'Connor, P. Roepstorff, N. L. Kelleher, T. D. Wood, F. W. McLafferty, and M. Mann, "Sequence Tag Identification of Intact Proteins by Matching Tandem Mass Spectral Data Against Sequence Data Bases", Proc. Natl. Acad. Sci. USA, 1996, 93, 8264-8267.
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  • C. A. Costello, N. L. Kelleher, M. Abe, F. W. McLafferty, and T. P. Begley, "Mechanistic Studies on Thiaminase I, Part 2: Overexpression and Identification of the Active Site Nucleophile", J. Biol. Chem., 1996, 271, 3445-3452.
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  • G. A. Valaskovic, N. L. Kelleher, D. P. Little, D. J. Aaseruud, and F. W. McLafferty, "Attomole-Sensitivity Electrospray Source for Large-Molecule Mass Spectrometry", Anal. Chem., 1995, 67, 3802-380.
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  • N. L. Kelleher, C. A. Costello, T. P. Begley, and F. W. McLafferty, Thiaminase I (42 kDa) Heterogeneity, Sequence Refinement, and Active Site Location from High-Resolution Tandem Mass Spectrometry", J. Am. Soc. Mass Spectrom., 1995, 6, 981-984.
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  • T. D. Wood, L. H. Chen, N. L. Kelleher, D. P. Little, G. L. Kenyon, and F. W. McLafferty, "Sequence Information from Heterogeneous Creatine Kinase (43 kDa) by High Resolution Tandem Mass Spectrometry", Biochemistry, 1995, 34, 16251-16254.
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  • F. W. McLafferty, M. W. Senko, D. P. Little, T. D. Wood, P. B. O'Connor, R. A. Chorush, and N. L. Kelleher, "Electrospray Ionization and Fourier-Transform Mass Spectrometry in Biomedical Research", Adv. Mass Spectrom., 1995, 13, 115-128.
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  • N. L. Kelleher, M. W. Senko, D. P. Little, P. B. O'Connor, and F. W. McLafferty, "Complete Large-Molecule High-Resolution Mass Spectra from 50 Femtomole Microvolume Injection", J. Am. Soc. Mass Spectrom., 1995, 6, 220-221.
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  • D. P. Little, R. A. Chorush, J. P. Speir, M. W. Senko, N. L. Kelleher, and F. W. McLafferty, "Rapid Sequencing of Oligonucleotides by High-Resolution Mass Spectrometry", J. Am. Chem. Soc. 1994, 116, 4893-4897.

Selected Invited Lectures (2000 - present; 52 total)

  • "Proteomics with 100% Sequence Coverage", UCLA Dept. of Chemistry and Biochemistry, Los Angeles, CA, January, 2000.
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  • "Toward Robust Detection of Post-translational Logic in Cells", MIT Whitehead Institute, Boston, MA, May, 2001.
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  • "Development of a General Platform for Robust Detection of Eukaryotic Post-translationalModifications: Separation Science, FT Mass Spectrometry, and Informatics", University of Michigan, Department of Chemistry, Ann Arbor, MI, November, 2001.
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  • "FT Mass Spectrometry for Detection of Covalent Intermediates in Polyketide and Non-ribosomal Peptide Biosynthesis", Bioorganic Gordon Conference, New Hampshire, June, 2002.
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  • "Translating the Potential of ESI and Q-FTMS for Top Down Proteomics into High Throughput Detection of All Post-Translational Modifications", Plenary Lecture for the 50th Anniversary of the Mass Spectrometry Society of Japan, RIKEN Institute, Tokyo, Japan, November, 2002.
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  • "Deciphering the Logic of Post-translational Modifications with Ultra-High Performance Mass Spectrometry", Pennsylvania State University, Department of Chemistry, December, 2002.
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  • "The First MS-Based System for Automated Tandem Mass Spectrometry Above 10 kDa", Northeast Region ACS Analytical Symposium , December, 2002.
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  • "Top Down Proteomics Driven by a New Quadrupole/Fourier-Transform Hybrid Mass Spectrometer", University of Georgia at Athens, Department of Chemistry, February, 2003.
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  • "Enzymology and Top Down Proteomics Spearheaded by Ultra-High Performance FT Mass Spectrometry", Department of Biochemistry and Biophysics, The University of Pennsylvania School of Medicine, March, 2003.
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  • "Decoding Biological Logic Written in the Language of Post-Translational Modification", University of Michigan, Life Sciences Initiative, April, 2003.
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  • "Automating the Analysis of Intact Proteins by High-Resolution Tandem FT Mass Spectrometry", American Society for Microbiology, May, 2003.
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  • "Top Down Proteomics of Saccharomyces cerevisiae driven by Q-FT Mass Spectrometry and Automated Nanospray", American Society of Mass Spectrometry National Meeting, Montreal, Canada, June, 2003.
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  • "Targeted Analysis and Discovery of Enzyme Post-Translational Modifications", Gordon Conference on Enzyme Mechanisms, July, 2003.
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  • "Deciphering the Logic of Post-translational Modifications with Ultra-High Performance Mass Spectrometry", Ohio State University, Dept. of Biochemistry and Biophysics, Columbus, OH, October, 2003.
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  • "The Evolution of Large Molecule Mass Spectrometry", Packard Foundation Annual Reunion, Vancouver, Canada, September, 2003.
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  • "New Approaches to Mass Spectrometry-Based Interrogation of Intact Proteins and Their Diverse Post-Translational Modifications", MIT, Department of Biological Engineering, Boston, MA, September, 2003.
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  • "Targeted Analysis and Discovery of Post-Translational Modifications by Top Down Mass Spectrometry", University of Texas at Austin, Department of Chemistry, October, 2003.
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  • "Of Histones and Codes: Parallels between Linkage Disequilibrium and Protein Characterization by Top Down Mass Spectrometry", MIT Center for Systems Biology Annual Symposium, Cambridge, MA, January, 2004.
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  • "Top Down Mass Spectrometry: 2004", Keystone Conference on Systems Biology, Santa Fe, NM, February, 2004.
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  • "Why a Chemist Needs Computer Science, Biology, and Engineering To Push the Limits of Modern Mass Spectrometry", Fermi Lab, Chicago, IL, February, 2004.
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  • "Top Down Proteomics: Has its time now come?", Plenary Lecture at the 4th Annual European Conference on Fourier-Transform Mass Spectrometry, University of Konstanz, Konstanz, Germany, March, 2004.
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  • "Top Down Proteomics Across the Phylogenetic Tree", 52nd American Society of Mass Spectrometry, Nashville, TN, May, 2004.

Technology Transfer

  • Established, trademarked, and filed two patents for several informatic tools and database search modes within a software suite called ProSight PTM.  It streamlines the identification and characterization of intact proteins and protein-based biomarkers based based on a sequenced genome or putative primary sequence. (https://prosightptm.scs.uiuc.edu) The web-based version of ProSight PTM now has ~200 external users (June, 2005).

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